Cyber Times International Journal of Technology & Management

In this paper, fermentation of damaged sorghum grains for bio-ethanol production is revealed by co-cultures of A. Niger (NCIM 1248) and S. Cerevisiae (MTCC 170). It is
observed that at 2% substrate concentration ethanol yield was 0.40 g/100ml, while ethanol concentration increased to a maximum of 1.81 g/100ml at 10% substrate concentration. When culture conditions were optimized, ethanol yield further increased to 1.91 g/100ml at a temperature of 350C, pH 5.0, 300 rpm agitation rate and reduced fermentation period of 5 days. In the present analysis of a simultaneous single step system for production of ethanol from damaged sorghum grains using symbiotic co-cultures of A. Niger and S. Cervisiae is carried out for determination of optimal culture conditions.