In present investigation the survey was carried out in Pune- Kolhapur region of Pune–Bangalore National highwayNo.-4.The road Pune to Kolhapur is a 4 lane highway for 225 km. and take 3hours and45 minutes to reach Kolhapur from Pune. It covers the major agriculture area of Pune, Satara, Sangli and Kolhapur district which may affect by vehicular pollution. The heavy metal tolerance in plants has received considerable attention by evolutionary biologists. The present investigation deals with the survey of roadside plants along Pune –Kolhapur highway. Survey of road is carried out by frequent visits to the locality. The plants observed are categorized under four sections as Roadside plants, Ornamental plants in lane dividers, Crop plants and Weed plants. Flora was dominated by13 families and 25 Sp. The dominant families observed are Caesalpiniaceae 3Sp., Apocynaceae 3 Sp., Verbinaceae 2Sp.., Mimoceae 2 Sp., Asteraceae 2 Sp., Poaceae 1Sp.,Euphorbiaceae 2 Sp., Bignoniaceae 1Sp., Meliaceae 1 Sp., Malvaceae 1 Sp., Plumbaginaceae 1 Sp., Fabaceae 1 Sp., Nyctaginaceae 1 Sp., While Dominant Caesalpinaceae And Apocynaceae represented By 3 Sp. each and remaining families are represented by 2 or 1 Sp. of each. Dominant Sp. of Ornamental plants in divider were Caesalpinia pulcherrima (L.) Sw., Duranta plumeri, Bougainvillia spectabilis willd, Tecoma stans H.B, K.Nov.,Cascabella thevetia (L.).,Nerium olender mill. , Plumbago zeylanica L.etc. Dominant trees observed along road side are Samanea saman (jacq.)merr. , Dalbergia sissoo roxb. ,Azadirachta indica juss. , Delonix regia (L.) gambl., Cassia fistula L.,etc. Dominant weed along road side and in divider are Alternanthera sessilis (L.)R. ,Euphorbia hirta L., Parthenium hysterophorus L.,Cynodon dactylon (L.) pers., Phyllanthus nururii Poir.., Tridax procumbens L.,Lantana camara L., etc. ,while wood plants such as Ficus bengalensis Linn.., Acacia nilotica (L)Willd., Pongamia glabra Ven.Jard.Malm.., etc,are also observed rarely Common crop plants are as Saccharum officinarum Linn.., Zea mays Linn.., Sorghum vulgare Pers., Oryza sativa L.,Triticum sativum Lam. Glycine max are observed seasonally.